BD Vacutainer™ Urine Specimen Collection

Principle

Urine collected into the BD Vacutainer ™ Specimen Collection Cup may be transferred via the integrated sampling device into an evacuated tube for transport and storage. In addition, the cup may be used to transport the entire specimen, provided adequate packaging and labeling is used.

Equipment, Reagents, Supplies and Quality Control Material

BD Vacutainer ™ Urine Complete Kit, Ref 364956
  1. One BD Vacutainer ™ PLUS Plastic C&S Preservative Tube with lyophilized maintenance formula for microbiology, 3 ml draw, sterile tube interior
  2. One BD Vacutainer ™ PLUS Plastic Conical UA Tube for urinalysis, 8 ml draw, sterile tube interior
  3. One graduated 4.5 oz screw-cap specimen cup with integrated sampling device, sterile interior
  4. Cleansing toweletes
BD C&S Transfer Straw Kit, Ref 364953, if applicable
  1. One BD Vacutainer ™ PLUS Plastic C&S Preservative Tube with lyophilized maintenance formula for microbiology, 3 ml draw, sterile tube interior
  2. Urine transfer straw, sterile

Collection Procedure

Patient Collect
  1. Remove the BD Vacutainer ™ tubes from a BD Vacutainer ™ Specimen Collection kit and place them in a protected location before giving the cup to the patient for urine collection.
  2. Give the cup to the patient and caution the patient not to remove the cap label. There is a “sharp” contained in the integrated sampling device.
  3. Instruct the patient on collection of a clean-voided, midstream urine specimen
  4. Instruct the patient to return the urine specimen to the health care professional immediately after collection.

Transfer of the specimen into the evacuated tube(s):

  1. Place cup upright on clean, flat surface. Container may be tipped at an angle if specimen volume is limited.
  2. Peel back label on cap to expose the integrated sampling device.
  3. The Urine C&S Preservative Tube (gray top) should be filled first when collecting multiple specimens, and must be filled to the minimum fill line on the tube(3 ml).
  4. Place the evacuated tube into the cavity on the cap with the stopper down. Advance the tube over the puncture point to pierce the stopper.
  5. Hold tube in position until filled. The tube vacuum will fill the tube with the desired volume.
  6. Remove tube from device.
  7. Shake C&S preservative tubes (gray top) vigorously to ensure complete dissolution of the preservative.
  8. Repeat steps 4-6 if a yellow tube is to be collected.
  9. Replace label over the integrated sampling device entrance hole and reseal.
  10. Label evacuated tubes for transport to the laboratory.
Using Transfer Straw
  1. Place collection container on a clean, flat surface.
  2. Submerge transfer straw into urine specimen. Container may be tipped at an angle if volume of urine is limited.
  3. The Urine C&S Preservative Tube (gray top) should be filled first when collecting multiple specimens, and must be filled to the minimum fill line on the tube(3 ml).
  4. Place evacuated tube into holder, stopper down. Advance the tube over puncture point to pierce stopper.
  5. Hold tube in position until filled.
  6. Remove tube from device.
  7. Shake C& S Preservative tube vigorously to ensure mixing.
  8. Remove tube from holder and set aside, leaving transfer straw in container.
  9. Repeat if another tube is to be collected.

Disposal

  1. The integrated sampling device caps are considered a 'sharp' and must be disposed of in an appropriate sharps container.
  2. If the cup is labeled with the patient's information, pour remaining urine in the dirty sink and dispose of the cup in a biohazard container. If the cup is unlabeled, dispose of in the regular trash.

Limitations

  1. There is no preservative present in the cup. Specimens not tested or preserved within 1-2 hours of collection should be refrigerated.
  2. Due to the deadspace in the BD Vacutainer ™ Specimen Collection Cup with Integrated sampling device, approximately 2.25 ml of the urine sample is unavailable in a cup resting on a flat surface. The cup may be tilted to fill the tube, and approximately 0.6 ml of the urine sample will be unavailable. If the sample volume in the cup is insufficient to fill the urine tube, remove the stopper of the tube and pour the urine into the tube. Tubes containing additives must be filled to the minimum fill lines.
  3. The urinalysis tubes that contain no preservative should be transported without delay to the laboratory for processing, or properly refrigerated to prevent erroneous results due to bacterial growth and/or specimen deterioration.
  4. The quantity of specimen drawn varies with altitude, ambient temperature, barometric pressure, tube age, and filling technique.
  5. Due to the instability of bilirubin and urobilinogen in urine when exposed to room temperature and light, testing should be performed as soon as possible or specimens should be stored in darkness.

Precautions

  1. Practice Standard Precautions. Use gloves, gowns, eye protection, other personal protective equipment, and engineering controls to protect from potential exposure to bloodborne pathogens.
  2. Transferring a sample collected using syringe and needle to a tube is not recommended. Additional manipulation of sharps such as hollow bore needles increases the potential for needlestick injury.
  3. Transferring a sample from syringe to an evacuated tube using non-sharps devices should be performed with caution: Do not depress the syringe plunger during transfer to avoid splatter and potential exposure. Do not overfill or under fill the tubes. Evacuated tubes are designed to draw the volume indicated. Filling is complete when vacuum no longer continues to draw.

References

BD Vacutainer ® Urine Products package insert, 7/04.

 

 

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